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( A, B ) The expression of Lac-FSVS (green in panel A) and E93-GFSTF (green in panel B) was observed at 15 and 24 hr after puparium formation (APF), respectively. Lac-FSVS was expressed in α′/β′ neurons (arrowheads) according to counter-staining with cell adhesion molecule Fasciclin II <t>(Fas2,</t> magenta in panel A ), which primarily labels γ neurons (arrows) at 15 hr APF. E93-GFSTF (double-arrows) was seen in the region with the weak RFP expression driven by GAL4-OK107 (magenta in panel B ). This pattern implies that F93-GFSTF expression occurs in the newly generated KCs, which are most likely α/β neurons, at 24 hr APF. Scale bar: 10 µm.
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mini circuits zaswa 2 50dra  (Mini-Circuits)
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( A, B ) The expression of Lac-FSVS (green in panel A) and E93-GFSTF (green in panel B) was observed at 15 and 24 hr after puparium formation (APF), respectively. Lac-FSVS was expressed in α′/β′ neurons (arrowheads) according to counter-staining with cell adhesion molecule Fasciclin II <t>(Fas2,</t> magenta in panel A ), which primarily labels γ neurons (arrows) at 15 hr APF. E93-GFSTF (double-arrows) was seen in the region with the weak RFP expression driven by GAL4-OK107 (magenta in panel B ). This pattern implies that F93-GFSTF expression occurs in the newly generated KCs, which are most likely α/β neurons, at 24 hr APF. Scale bar: 10 µm.
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( A, B ) The expression of Lac-FSVS (green in panel A) and E93-GFSTF (green in panel B) was observed at 15 and 24 hr after puparium formation (APF), respectively. Lac-FSVS was expressed in α′/β′ neurons (arrowheads) according to counter-staining with cell adhesion molecule Fasciclin II <t>(Fas2,</t> magenta in panel A ), which primarily labels γ neurons (arrows) at 15 hr APF. E93-GFSTF (double-arrows) was seen in the region with the weak RFP expression driven by GAL4-OK107 (magenta in panel B ). This pattern implies that F93-GFSTF expression occurs in the newly generated KCs, which are most likely α/β neurons, at 24 hr APF. Scale bar: 10 µm.
Non Esterified Fatty Acids Nefa, supplied by Randox, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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plasma nefa concentrations  (Randox)
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( A, B ) The expression of Lac-FSVS (green in panel A) and E93-GFSTF (green in panel B) was observed at 15 and 24 hr after puparium formation (APF), respectively. Lac-FSVS was expressed in α′/β′ neurons (arrowheads) according to counter-staining with cell adhesion molecule Fasciclin II <t>(Fas2,</t> magenta in panel A ), which primarily labels γ neurons (arrows) at 15 hr APF. E93-GFSTF (double-arrows) was seen in the region with the weak RFP expression driven by GAL4-OK107 (magenta in panel B ). This pattern implies that F93-GFSTF expression occurs in the newly generated KCs, which are most likely α/β neurons, at 24 hr APF. Scale bar: 10 µm.
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( A, B ) The expression of Lac-FSVS (green in panel A) and E93-GFSTF (green in panel B) was observed at 15 and 24 hr after puparium formation (APF), respectively. Lac-FSVS was expressed in α′/β′ neurons (arrowheads) according to counter-staining with cell adhesion molecule Fasciclin II <t>(Fas2,</t> magenta in panel A ), which primarily labels γ neurons (arrows) at 15 hr APF. E93-GFSTF (double-arrows) was seen in the region with the weak RFP expression driven by GAL4-OK107 (magenta in panel B ). This pattern implies that F93-GFSTF expression occurs in the newly generated KCs, which are most likely α/β neurons, at 24 hr APF. Scale bar: 10 µm.
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( A, B ) The expression of Lac-FSVS (green in panel A) and E93-GFSTF (green in panel B) was observed at 15 and 24 hr after puparium formation (APF), respectively. Lac-FSVS was expressed in α′/β′ neurons (arrowheads) according to counter-staining with cell adhesion molecule Fasciclin II <t>(Fas2,</t> magenta in panel A ), which primarily labels γ neurons (arrows) at 15 hr APF. E93-GFSTF (double-arrows) was seen in the region with the weak RFP expression driven by GAL4-OK107 (magenta in panel B ). This pattern implies that F93-GFSTF expression occurs in the newly generated KCs, which are most likely α/β neurons, at 24 hr APF. Scale bar: 10 µm.
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zfa fmk  (MedChemExpress)
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( A, B ) The expression of Lac-FSVS (green in panel A) and E93-GFSTF (green in panel B) was observed at 15 and 24 hr after puparium formation (APF), respectively. Lac-FSVS was expressed in α′/β′ neurons (arrowheads) according to counter-staining with cell adhesion molecule Fasciclin II <t>(Fas2,</t> magenta in panel A ), which primarily labels γ neurons (arrows) at 15 hr APF. E93-GFSTF (double-arrows) was seen in the region with the weak RFP expression driven by GAL4-OK107 (magenta in panel B ). This pattern implies that F93-GFSTF expression occurs in the newly generated KCs, which are most likely α/β neurons, at 24 hr APF. Scale bar: 10 µm.
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s7023 zfa fmk medchemexpress  (MedChemExpress)
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( A, B ) The expression of Lac-FSVS (green in panel A) and E93-GFSTF (green in panel B) was observed at 15 and 24 hr after puparium formation (APF), respectively. Lac-FSVS was expressed in α′/β′ neurons (arrowheads) according to counter-staining with cell adhesion molecule Fasciclin II <t>(Fas2,</t> magenta in panel A ), which primarily labels γ neurons (arrows) at 15 hr APF. E93-GFSTF (double-arrows) was seen in the region with the weak RFP expression driven by GAL4-OK107 (magenta in panel B ). This pattern implies that F93-GFSTF expression occurs in the newly generated KCs, which are most likely α/β neurons, at 24 hr APF. Scale bar: 10 µm.
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nefa concentrations  (Randox)
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( A, B ) The expression of Lac-FSVS (green in panel A) and E93-GFSTF (green in panel B) was observed at 15 and 24 hr after puparium formation (APF), respectively. Lac-FSVS was expressed in α′/β′ neurons (arrowheads) according to counter-staining with cell adhesion molecule Fasciclin II <t>(Fas2,</t> magenta in panel A ), which primarily labels γ neurons (arrows) at 15 hr APF. E93-GFSTF (double-arrows) was seen in the region with the weak RFP expression driven by GAL4-OK107 (magenta in panel B ). This pattern implies that F93-GFSTF expression occurs in the newly generated KCs, which are most likely α/β neurons, at 24 hr APF. Scale bar: 10 µm.
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radiofrequency switch  (Mini-Circuits)
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( A, B ) The expression of Lac-FSVS (green in panel A) and E93-GFSTF (green in panel B) was observed at 15 and 24 hr after puparium formation (APF), respectively. Lac-FSVS was expressed in α′/β′ neurons (arrowheads) according to counter-staining with cell adhesion molecule Fasciclin II <t>(Fas2,</t> magenta in panel A ), which primarily labels γ neurons (arrows) at 15 hr APF. E93-GFSTF (double-arrows) was seen in the region with the weak RFP expression driven by GAL4-OK107 (magenta in panel B ). This pattern implies that F93-GFSTF expression occurs in the newly generated KCs, which are most likely α/β neurons, at 24 hr APF. Scale bar: 10 µm.
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Image Search Results


( A, B ) The expression of Lac-FSVS (green in panel A) and E93-GFSTF (green in panel B) was observed at 15 and 24 hr after puparium formation (APF), respectively. Lac-FSVS was expressed in α′/β′ neurons (arrowheads) according to counter-staining with cell adhesion molecule Fasciclin II (Fas2, magenta in panel A ), which primarily labels γ neurons (arrows) at 15 hr APF. E93-GFSTF (double-arrows) was seen in the region with the weak RFP expression driven by GAL4-OK107 (magenta in panel B ). This pattern implies that F93-GFSTF expression occurs in the newly generated KCs, which are most likely α/β neurons, at 24 hr APF. Scale bar: 10 µm.

Journal: eLife

Article Title: Genetic network shaping Kenyon cell identity and function in Drosophila mushroom bodies

doi: 10.7554/eLife.108173

Figure Lengend Snippet: ( A, B ) The expression of Lac-FSVS (green in panel A) and E93-GFSTF (green in panel B) was observed at 15 and 24 hr after puparium formation (APF), respectively. Lac-FSVS was expressed in α′/β′ neurons (arrowheads) according to counter-staining with cell adhesion molecule Fasciclin II (Fas2, magenta in panel A ), which primarily labels γ neurons (arrows) at 15 hr APF. E93-GFSTF (double-arrows) was seen in the region with the weak RFP expression driven by GAL4-OK107 (magenta in panel B ). This pattern implies that F93-GFSTF expression occurs in the newly generated KCs, which are most likely α/β neurons, at 24 hr APF. Scale bar: 10 µm.

Article Snippet: Primary antibodies used in this study included guinea pig antibody against Chinmo (1:1000, Sokol laboratory ), rat monoclonal antibody against mCD8 (1:100, Thermo Fisher Scientific), rabbit antibody against GFP (1:750, Thermo Fisher Scientific), and mouse monoclonal antibodies against EcR-B1 (1:50, DSHB), Fas2 (1:100, DSHB) and Trio (1:50, DSHB).

Techniques: Expressing, Staining, Generated

( A, B ) Overexpression of E93 driven by GAL4-OK107 caused precocious expression of α/β-specific Ca-α1T-GFSTF in early-born KCs at the wandering larval (WL) stage. ( C, D ) In addition, overexpression of E93 driven by a γ-neural driver, GAL4-201Y (magenta), ectopically turned on the expression of a α/β-specific 70F05-LexA driver in a portion of γ neurons (visualized by myr-GFP in green; arrow). On the other hand, overexpression of E93 abolished γ-specific markers, including Ab-GFP ( E, F ), Mamo H/I ( G, H ), Mamo D~G (weak green signal; I, J ) and EcR-B1 ( E–J ), and α′/β′-specific Mamo D~G (strong green signal within yellow dashed-line; I, J ) in the early-born KCs at the white pupal (WP) stage. ( K, L ) E93 overexpression also compromised the Lac-FSVS expression in α′/β′ neurons and the morphology of mushroom body (MB) lobes revealed by cell adhesion molecule Fasciclin II (Fas2, strong magenta for labeling α and β lobes) at 24 hr after puparium formation (APF). An enhance-promoter (EP) line inserted at the proximal region of the E93-A 5′UTR was used to overexpress E93 in the gain-of-function experiments. The potency of the E93(EP) line was similar to two other in-house transgenic lines expressing E93-A and E93-B isoforms (see ). Scale bar: 10 µm.

Journal: eLife

Article Title: Genetic network shaping Kenyon cell identity and function in Drosophila mushroom bodies

doi: 10.7554/eLife.108173

Figure Lengend Snippet: ( A, B ) Overexpression of E93 driven by GAL4-OK107 caused precocious expression of α/β-specific Ca-α1T-GFSTF in early-born KCs at the wandering larval (WL) stage. ( C, D ) In addition, overexpression of E93 driven by a γ-neural driver, GAL4-201Y (magenta), ectopically turned on the expression of a α/β-specific 70F05-LexA driver in a portion of γ neurons (visualized by myr-GFP in green; arrow). On the other hand, overexpression of E93 abolished γ-specific markers, including Ab-GFP ( E, F ), Mamo H/I ( G, H ), Mamo D~G (weak green signal; I, J ) and EcR-B1 ( E–J ), and α′/β′-specific Mamo D~G (strong green signal within yellow dashed-line; I, J ) in the early-born KCs at the white pupal (WP) stage. ( K, L ) E93 overexpression also compromised the Lac-FSVS expression in α′/β′ neurons and the morphology of mushroom body (MB) lobes revealed by cell adhesion molecule Fasciclin II (Fas2, strong magenta for labeling α and β lobes) at 24 hr after puparium formation (APF). An enhance-promoter (EP) line inserted at the proximal region of the E93-A 5′UTR was used to overexpress E93 in the gain-of-function experiments. The potency of the E93(EP) line was similar to two other in-house transgenic lines expressing E93-A and E93-B isoforms (see ). Scale bar: 10 µm.

Article Snippet: Primary antibodies used in this study included guinea pig antibody against Chinmo (1:1000, Sokol laboratory ), rat monoclonal antibody against mCD8 (1:100, Thermo Fisher Scientific), rabbit antibody against GFP (1:750, Thermo Fisher Scientific), and mouse monoclonal antibodies against EcR-B1 (1:50, DSHB), Fas2 (1:100, DSHB) and Trio (1:50, DSHB).

Techniques: Over Expression, Expressing, Labeling, Transgenic Assay